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ATCC
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ATCC
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ATCC
gram positive bacteria Gram Positive Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/gram positive bacteria/product/ATCC Average 99 stars, based on 1 article reviews
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ATCC
được sàng lọc bước đầu bằng phương pháp khuếch tán trên đĩa thạch chống lại 9 mầm bệnh bao gồm vi khuẩn gram dương được Sàng Lọc Bước đầu Bằng Phương Pháp Khuếch Tán Trên đĩa Thạch Chống Lại 9 Mầm Bệnh Bao Gồm Vi Khuẩn Gram Dương, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/được sàng lọc bước đầu bằng phương pháp khuếch tán trên đĩa thạch chống lại 9 mầm bệnh bao gồm vi khuẩn gram dương/product/ATCC Average 99 stars, based on 1 article reviews
được sàng lọc bước đầu bằng phương pháp khuếch tán trên đĩa thạch chống lại 9 mầm bệnh bao gồm vi khuẩn gram dương - by Bioz Stars,
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ATCC
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ATCC
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LGC Standards
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Addgene inc
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Merck KGaA
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siTools Biotech
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Image Search Results
Journal: bioRxiv
Article Title: Destabilized 3’UTR ARE therapeutically degrades ERBB2 in drug-resistant ERBB2+ cancer models
doi: 10.1101/2022.08.14.503914
Figure Lengend Snippet: A. Dot blots show the phospho kinase array pattern of the BT474 clone 5 wild type, BT474 clone 5 Vector, BT474 clone 5 desARE3’UTR ERBB2-3 and BT474 clone 5 desARE3’UTR c-MYC 2-3. The dot marked in red box show unique kinases down regulated only in BT474 clone 5 desARE3’UTR ERBB2-3 and the dot marked in green boxes show unique kinases downregulated only in BT474 clone 5 desARE3’UTR c-MYC 2-3. Each kinase is spotted twice. B. The bar chart shows the intensity of the phospho-kinase array dot blot for the BT474 clone 5 wild type (black bar), BT474 clone 5 Vector (blue bar), BT474 clone 5 desARE3’UTR ERBB2-3 (red bar) and BT474 clone 5 desARE3’UTR c-MYC 2-3 (green bar). C. The bar charts show YES1 pT60 and WNK1 pT60 kinases specifically down regulated in BT474 clone 5 desARE3’UTR ERBB2-3 in red bar. Controls BT474 clone 5 wild type (black bar), BT474 clone 5 Vector (blue bar), and BT474 clone 5 desARE3’UTR c-MYC 2-3 (green bar). T-test (*** Pval=0.00413, desARE3’UTRERBB2-3 for YES and WNK1) D. Venn diagram show kinases downregulated in destabilized desARE3’UTR ERBB2-3, 30 NCI-H1975 (p=0.02) blue (from RNA Seq), and in destabilized desARE3’UTR ERBB2-3 BT474 clone 5 (red-kinase array) and the shared kinases found downregulated in destabilized ERBB2 in both resistant cell lines WNK1 and YES1 in pink. E. Western blot show YES1 and GAPDH expression across the NCI-H1975 wildtype cells, vector, cells containing constructs desARE3’UTR ERBB2-1, 3 and 30 and wild type trastuzumab treated NCI-H1975 cells (n=2). F. Bar chart show quantification of the YES1 expression normalized against GAPDH on NCI-H1975 wildtype cells, vector and cells containing constructs desARE3’UTR ERBB2-1, 3 and 30 (n=2). T-test (***Pval=0.0021, desARE3’UTRERBB2-1,3,30) G. Western blot show WNK1 and GAPDH expression across the NCI-H1975 wildtype cells, vector, cells containing constructs desARE3’UTR ERBB2-1, 3 and 30 and wild type trastuzumab treated NCI-H1975 cells. H. Western blot show YES1 and GAPDH expression across the NCI-H1975 destabilized with constructs desARE3’UTR ERBB2-3 and 30 (lane 1 and 3) and over expressed with YES1 in lane 2 and 4. I. Bar chart show the wound healing closing of NCI H1975 wildtype, vector, desARE3’UTRERBB2-1, 3,30 and desARE3’UTR ERBB2-30,30 overexpressed with YES1 from 0hr to 72hrs. At 24hrs *** p=0.00025 desARE3’UTRERBB2-3,30. At 48hrs ****p=0.000018 desARE3’UTR ERBB2-3 and at 72hrs *** p=0.0003 desARE3’UTRERBB2-3 and **p=0.002 desARE3’UTRERBB2-30.
Article Snippet:
Techniques: Plasmid Preparation, Dot Blot, RNA Sequencing, Western Blot, Expressing, Construct
Journal: bioRxiv
Article Title: Destabilized 3’UTR ARE therapeutically degrades ERBB2 in drug-resistant ERBB2+ cancer models
doi: 10.1101/2022.08.14.503914
Figure Lengend Snippet: A. Microscope images of wound healing assay of NCI H1975 WT, vector, desARE3’UTRERBB2-1, 3 and 30 and desARE3’UTRERBB2-3 and 30 overexpressed with YES1.Images were taken at 0hr, 24hr, 48hrs and 72hrs at 10x.
Article Snippet:
Techniques: Microscopy, Wound Healing Assay, Plasmid Preparation